Cell Senescence Entries for AAK1

Cell Types
Embryonic kidney, Lung fibroblast
Cell Lines
HEK293T, IMR-90, MRC-5
Cancer Cell?
No
Method
Overexpression
Type of senescence
Replicative
Senescence Effect
Induces
Observations
A library of 200 constitutively activated kinases was screened in order to identify kinases whose constitutive expression decreased cell proliferation and induced expression of senescence markers; p16 and SASP components. A total of 33 kinases showed pro-senescence effects (particularly MAP3K7, PRKCD, and MATK); kinases with strong pro-senescence effects, induced expression of SASP-component genes (e.g. IL1A, IL1B, IL6 and IL8) through activation of an NF-kappaB-dependent transcriptional program. Inhibition of the NF-kappaB, p53, or Rb pathway proved insufficient to prevent kinase-triggered cell cycle arrest.
Primary Reference

    AAK1 Gene Information

    HGNC symbol
    AAK1 
    Aliases
     
    Common name
    AP2 associated kinase 1 
    Entrez Id
    22848
    Description
    Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

    AAK1 Ontologies

    Gene Ontology
    Process: GO:6897; endocytosis
    GO:61024; membrane organization
    GO:45747; positive regulation of Notch signaling pathway
    GO:46777; protein autophosphorylation
    GO:6468; protein phosphorylation
    GO:50821; protein stabilization
    GO:2000369; regulation of clathrin-dependent endocytosis
    GO:32880; regulation of protein localization
    Cellular component: GO:31252; cell leading edge
    GO:5905; clathrin-coated pit
    GO:30136; clathrin-coated vesicle
    GO:5737; cytoplasm
    GO:5829; cytosol
    GO:19897; extrinsic component of plasma membrane
    GO:43195; terminal bouton
    Function: GO:35612; AP-2 adaptor complex binding
    GO:5524; ATP binding
    GO:5112; Notch binding
    GO:5515; protein binding
    GO:4674; protein serine/threonine kinase activity
    Show all GO terms

    Homologs of AAK1 in Model Organisms

    Caenorhabditis elegans
    sel-5
    Danio rerio
    aak1b
    Danio rerio
    aak1a
    Drosophila melanogaster
    Nak
    Mus musculus
    Aak1
    Rattus norvegicus
    Aak1
    Saccharomyces cerevisiae
    ARK1
    Saccharomyces cerevisiae
    AKL1
    Saccharomyces cerevisiae
    PRK1

    In other databases

    GenAge model organism genes
    • A homolog of this gene for Caenorhabditis elegans is present as sel-5
    • A homolog of this gene for Saccharomyces cerevisiae is present as AKL1
    • A homolog of this gene for Saccharomyces cerevisiae is present as PRK1
    • A homolog of this gene for Saccharomyces cerevisiae is present as ARK1

    External links

    OMIM
    616405
    Ensembl
    ENSG00000115977
    Entrez Gene
    22848
    UniGene
    468878
    1000 Genomes
    1000 Genomes
    HPRD
    GenAtlas
    AAK1
    GeneCards
    AAK1